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Analyses of Bone Regeneration Capacity of Freeze-Dried Bovine Bone and Combined Deproteinized–Demineralized Bovine Bone Particles in Mandibular Defects: The Potential Application of Biological Forms of Bovine-Bone Filler
Journal
European Journal of Dentistry
ISSN
1305-7456
1305-7464
Date Issued
2021
Author(s)
David Buntoro Kamadjaja
Handhito Satriyo
Aris Setyawan
Yeni Dian Lesmaya
Jefry Wahyudi Safril
Ni Putu Mira Sumarta
Andra Rizqiawan
Coen Pramono Danudiningrat
Ta To Tran
DOI
10.1055/s-0041-1736291
Abstract
Objective This study aimed to evaluate bone regeneration capacity of FDBX granules compared to composite DBBM/DFDBX granules for filling of bone defect in rabbit mandible.
Material and Methods Critical size defects were created in 45 rabbits' mandible. The defect in the control group is left untreated, while in other groups the defects were filled with FDBX granules and composite DBBM/DFDBX granules, respectively. Specimens were collected at 2, 4, and 8 weeks for histology and immunohistochemical analyses. Significant difference is set at p-value < 0.05.
Results The osteoblast-osteoclast quantification, osteoblast expression of Runx2, alkaline phosphatase, collagen-I, and osteocalcin, and osteoclast expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in FDBX groups were statistically comparable (p > 0.05) with the composite group, while OPG/RANKL ratio, bone healing scores, and trabecular area were significantly higher (p < 0.05) in the composite compared to FDBX group.
Conclusion Composite DBBM/DFDBX granules, within the limitation of this study, has better bone forming capacity than FDBX granules for filling of bone defects in the mandible.
Material and Methods Critical size defects were created in 45 rabbits' mandible. The defect in the control group is left untreated, while in other groups the defects were filled with FDBX granules and composite DBBM/DFDBX granules, respectively. Specimens were collected at 2, 4, and 8 weeks for histology and immunohistochemical analyses. Significant difference is set at p-value < 0.05.
Results The osteoblast-osteoclast quantification, osteoblast expression of Runx2, alkaline phosphatase, collagen-I, and osteocalcin, and osteoclast expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in FDBX groups were statistically comparable (p > 0.05) with the composite group, while OPG/RANKL ratio, bone healing scores, and trabecular area were significantly higher (p < 0.05) in the composite compared to FDBX group.
Conclusion Composite DBBM/DFDBX granules, within the limitation of this study, has better bone forming capacity than FDBX granules for filling of bone defects in the mandible.
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