Synthesis of Poly (Styrene Sulfonate) Coated Gd3+ Poly (Lactide Co Glycolic Acid) Perfluoro Bromide Nanoparticles for Stem Cell Monitoring Using Magnetic Resonance Imaging

Magnetic resonance imaging ( is one of best imaging technologies to monitor
transplanted stem cells, because of its high anatomical resolution and safety. In this study, we aim to
synthesize Poly (styrene sulphonate) coated Gd 3+ Poly (lactide co glycolic acid) Perfluorooctyl
Bromide nanoparticles (PSS coated Gd 3+3+@ for stem cell labeling using both 1 H and 19 F MRI. At a
weight ratio 20 of PLGA/ Gd 3+ NPs have a spherical shape with the hydrodynamic siz e at 180 nm with
zeta potential at 53 mV. In vitro we obtain the intended T1 relaxation acceleration of water on 1 H MRI.
The NPs have low toxicity to the human mesenchymal stem cells ( up to 1 mg/ml. They could
internalize into the stem cells via ca veolae mediated endocytosis efficiently, confirmed by flow cytometry
analysis, and gives a good contrast in both channels 1 H and 18 F MRI scans. In conclusion, our NPs have
shown a great potential as a dual contrast agent in MRI for stem cell monitoring.