Thuc-Huy DuongHung-Huy NguyenThanh-Trung LeThanh-Nha TranJirapast SichaemThanh-Trung NguyenThi-Phuong NguyenDinh-Tri MaiHuu-Hung NguyenHoang-Duy Le2022-11-032022-11-032020https://repository.vlu.edu.vn/handle/123456789/83810.1016/j.fitote.2020.104512Chemical investigation of the cultured polyspore-derived mycobionts of a Pseudopyrenula subnudata lichen led to the isolation of two new compounds, subnudatones A and B (1 and 2), together with four known compounds, 1- (2-hydroxy-1,2,6-trimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen-1-yl)ethanone (3), libertalide C (4), aspermytin A (5), and 6,7-dimethoxy-4-hydroxymellin (6). Their chemical structures were elucidated by extensive 1D and 2D NMR analysis and high resolution mass spectroscopy, and comparisons were made with the literature. The absolute configuration of 1 was defined unambiguously using single crystal X-ray crystallography. Compound 1 represents the first dimeric decalin polyketide to be found in nature. The in vitro cytotoxicity of 1 against two cancer cell lines (K562 and MCF-7) was evaluated. Compound 1 showed moderate cytotoxic activity with IC50 values of 23.5 ± 1.0 and 51.9 ± 1.4 μM, respectively.en-US"Pseudopyrenula subnudataSubnadatones A and Btrans-decalin polyketideCytotoxicity"journal-article